تسجيل الدخول

تسجيل

Microparticle methods

المؤلف:  Sue Jickells , Adam Negrusz (Editors)

المصدر:  Clarkes Analytical Forensic Toxicology

الجزء والصفحة:  388

2026-11-05

126

Microparticle methods

Agglutination of microparticles has been used as a signal system in homogeneous assay-based methods. Agglutination reactions for drugs use particles, generally latex, that have drug conjugated onto them. Addition of antibody (which has two binding sites) to the drug-coated particles causes bridging between antibodies and the particles to produce agglutinates. The drug present in a sample, added to the mixture of anti-body and drug-conjugated particles, competes for antibody-binding sites and therefore prevents agglutination. In this type of assay, the degree of agglutination is inversely proportional to the amount of drug in the sample.

The basis of the Roche Abuscreen Online system termed KIMS (kinetic interaction of microparticles in solution) involves monitoring the rate of agglutination via spectrophotometric means.