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Validity of the Beer–Lambert law

المؤلف:  Sue Jickells , Adam Negrusz (Editors)

المصدر:  Clarkes Analytical Forensic Toxicology

الجزء والصفحة:  397

2026-11-07

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Validity of the Beer–Lambert law

The validity of the Beer–Lambert law is affected by a number of factors. If the radiation is nonmonochromatic, that is its spectral band width is greater than about 10% of the drug absorption bandwidth at half-height, the observed absorbance will be lower than the ‘true’ limiting value for monochromatic radiation. Thus, sharp bands are more susceptible than broad bands to absorbance error on this account. Moreover, if the absorbing species is nonhomogeneous, or if it undergoes association, dissociation, photodegradation, solvation, complexation or adsorption, or if it emits fluorescence, then positive or negative deviations from the Beer–Lambert law may be observed. Stray-light effects and the type of solvent used may also lead to noncompliance with the Beer–Lambert law.