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تسجيل

Coupled techniques

المؤلف:  Sue Jickells , Adam Negrusz (Editors)

المصدر:  Clarkes Analytical Forensic Toxicology

الجزء والصفحة:  p414-415

2026-11-10

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Coupled techniques

As with UV-visible detectors which are coupled to HPLC, for components of interest that fluoresce, the chromatographic system can be equipped with a fluorimetric detector (LC-FL). In the case of weakly fluorescent or nonfluorescent drugs, a number of well-characterised derivatisation reactions are available. These include dansyl chloride (5-dimethylaminonaphthalene 1-sulfonyl chloride) for primary and secondary amines (Fig.1) and phenolichydroxylgroups, and fluorescamine 4-phenylspiro-[furan-2(3H), 1-isobenzofuran]-3,3-dione, fluorogenic 2,1,3 benzoxadiazoles and o-phthalaldehyde for primary amines. Derivatisation reactions of this type have extended the scope of fluorescence detection in HPLC significantly. A fluorescence detector can be coupled with capillary electrophoresis (CE-FL) or ion chromatography to determine charged components in complex mixtures.

Figure 1 Example of derivatisation to produce a fluorescent derivative. (A) Derivatization of an amino acid using NBD-F. (B) Derivatisation of a primary amine using dansyl chloride.